Cathepsin D antibody

Catalog No. ABIN783842

The Rabbit Polyclonal anti-Cathepsin D antibody is suitable to detect Cathepsin D in samples from Human, Mouse and Rat. It has been validated for WB and IP.

Quick Overview for Cathepsin D antibody (ABIN783842)

Target: Cathepsin D (CTSD)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cathepsin D antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-Cathepsin D Antibody

Specificity: This antibody detects both the 52 kDa proform and the 34 kDa cleaved fragment of Cathepsin D.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Affinity chromatography

Immunogen: Synthetic peptide surrounding amino acid 140 of mouse cathepsins D

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, 0.01 % Thimerosal, 30 % Glycerol, 0.5 % BSA

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Upon receipt, store (in aliquots) at -20 to -70 °C.

Target Details for Cathepsin D

Target: Cathepsin D (CTSD)

Alternative Name: Cathepsin D

Background: Cathepsin D is a normal lysosomal protease that is expressed in all cells. It is an aspartyl protease with a pH optimum in the range of 3-5, and contains two N-linked oligosaccharides. Cathepsin D is synthesized as an inactive 52 kDa pro-enzyme. Activation involves the proteolytic removal of the 43 amino acid profragment and an internal cleavage to generate the two-chain form made up of 34 and 14 kDa subunits. Cathepsin D contains the mannose-6-phosphate lysosomal localization signal that targets the enzyme to the lysosomal compartment where it functions in the normal degradation of proteins. In certain tumor cells, Cathepsin D is abnormally processed and is secreted in its 52 kDa precursor form. Numerous clinical studies as well as in vitro evidence suggest that cathepsin D plays an important role in malignant transformation and may be a useful prognostic indicator for breast cancer.Synonyms: CPSD, CTSD

Gene ID: 1509

NCBI Accession: NP_001900

UniProt: P07339

Pathways: Peptide Hormone Metabolism