ENOS antibody (AA 1181-1194)

Catalog No. ABIN784232

The Rabbit Polyclonal anti-ENOS antibody is suitable to detect ENOS in samples from Human, Rat, Mouse, Pig, Cow, Dog and Guinea Pig. It has been validated for WB and IHC (p).

Quick Overview for ENOS antibody (AA 1181-1194) (ABIN784232)

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reactivity: Human, Rat, Mouse, Pig, Cow, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENOS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ENOS Antibody

Binding Specificity: AA 1181-1194

Sequence: CQERQLRGAV PWAFD Remarks: The immunizing sequence has 10/11 identical amino acids in Mouse, Guinea Pig, Bovine, Porcine, Rat and Canine. Antigen Modificati on: aa1181-1194

Specificity: Recognizes eNOS/NOS III, Mr ~135 kDa.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Dog, Guinea pig, Mouse, Porcine, Rat.
Species reactivity (tested):Human.

Purification: Protein A Chromatography.

Immunogen: kLH-conjugated, synthetic peptide corresponding to amino acids 1181-1194 of Human eNOS/NOS III.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.6, 0.07 % Sodium Azide, before the addition of Glycerol to 30 %.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Alternative Name: NOS3

Background: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene.Synonyms: Constitutive NOS, EC-NOS, Endothelial NOS, NOS type III, Nitric oxide synthase, eNOS, endothelial

Gene ID: 4846

NCBI Accession: NP_000594

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling