Tryptophan Hydroxylase 2 antibody (N-Term)

Catalog No. ABIN784279

This Goat Polyclonal antibody specifically detects Tryptophan Hydroxylase 2 in WB and EIA. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow and Pig.

Quick Overview for Tryptophan Hydroxylase 2 antibody (N-Term) (ABIN784279)

Target: Tryptophan Hydroxylase 2 (TPH2)

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Tryptophan Hydroxylase 2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Tryptophan Hydroxylase 2 Antibody

Binding Specificity: AA 16-29, N-Term

Sequence: RGFSLDSAVP EEHQ-C

Specificity: Recognizes TPH2 (16-29).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Pig, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the N Terminus of the protein sequence according to NP_775489.2. Genename: TPH2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Tryptophan Hydroxylase 2

Target: Tryptophan Hydroxylase 2 (TPH2)

Alternative Name: Tryptophan 5-Hydroxylase 2 (TPH2)

Background: Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000, Johansen et al., 1996). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2 (McKinney et al., 2005).Synonyms: NTPH, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2

Gene ID: 121278

NCBI Accession: NP_775489