Albumin antibody (HRP)

Catalog No. ABIN784429

This Rabbit Polyclonal antibody specifically detects Albumin in WB, IF, ID, RIA and IHC (fro). It exhibits reactivity toward Rat.

Quick Overview for Albumin antibody (HRP) (ABIN784429)

Target: Albumin (ALB)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Albumin antibody is conjugated to HRP

Application: Western Blotting (WB), Immunofluorescence (IF), Immunodiffusion (ID), Radioimmunoassay (RIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Albumin Antibody (HRP)

Specificity: Tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal rat serum and purified rat albumin. One characteristic precipitin line is obtained against pooled normal rat serum using different antigen/antibody concentration ratio's. Precipitin lines against normal rat serum and purified rat albumin give a reaction of full identity. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has been tested for cross-reactivity by double radial immunodiffusion against several species sera with the following results: ++ mouse, guinea pig + monkey ± horse, human, swine - rabbit, sheep, goat, canine, chicken, bovine

Characteristics: Molar Ratio: 1,7

Purification: DEAE-column Chromatography

Immunogen: Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from rat serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 1 mL sterile distilled water

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Albumin

Background: Synonyms: ALB, BSA, HSA, Serum Albumin

Gene ID: 24186

NCBI Accession: NP_599153

Pathways: Lipid Metabolism