Albumin antibody (HRP)

Catalog No. ABIN784493

The Goat Polyclonal anti-Albumin antibody is suitable to detect Albumin in samples from Rat. It has been validated for WB, IP, IF, EIA, RIA and IHC (fro).

Quick Overview for Albumin antibody (HRP) (ABIN784493)

Target: Albumin (ALB)

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Albumin antibody is conjugated to HRP

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Albumin Antibody (HRP)

Specificity: Tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal rat serum and purified rat albumin. One characteristic precipitin line is obtained against pooled normal rat serum using different antigen/antibody concentration ratio's. Precipitin lines against normal rat serum and purified rat albumin give a reaction of full identity. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion against several species sera with the following results: - bovine, goat, chicken and sheep ± rabbit + dog, horse, human, monkey and swine ++ guinea pig and mouse A negative reaction in double radial immunodiffusion does not exclude a weak positive reaction in more sensitive techniques.

Characteristics: Molar Ratio: 1,2

Purification: DEAE-column Chromatography

Immunogen: Purified albumin isolated from pooled rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 1 mL sterile distilled water.

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Albumin

Background: Synonyms: ALB, BSA, HSA, Serum Albumin

Gene ID: 24186

NCBI Accession: NP_599153

Pathways: Lipid Metabolism