TRF1 antibody

Catalog No. ABIN790393

This Mouse Monoclonal antibody specifically detects TRF1 in ELISA, IHC, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for TRF1 antibody (ABIN790393)

Target: TRF1 (TERF1) (Telomeric Repeat Binding Factor (NIMA-Interacting) 1 (TERF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRF1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: BED5

Product Details anti-TRF1 Antibody

Specificity: Recognizes TTAGGG repeat binding factor 1 (TRF1) a ubiquitously expressed 60kD protein. Along with tankyrase-1, TIN2, and PINX1, TRF1 binds the double-stranded TTAGGG repeat of telomeres and regulates telomere length. Protection of telomeres-1 (POT1) is a single-stranded telomeric DNA-binding protein which controls the elongation of telomeres by telomerase. TRF1 mediates the loading of POT1 onto single stranded DNA, conveying information about telomere length to the telomere terminus, where telomerase is regulated. If the telomere is long enough, telomerase is inhibited. Additionally in conjunction with POT1 and TTAGGG repeat binding factor 1 (TRF2), TRF1 recruits factors to the telomere, inducing the formation of the protective t-loop. TRF1 along with TRF2, POT1, TIN2, TPP1, and Rap1 form the shelterin complex which distinguishes telomeres from sites of DNA damage. Reported to work in Western blotting applications.

Purification: Affinity purified

Immunogen: Recombinant TRF1

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer  pH 6.0 is recommended for this purpose Histology Positive Control Tissue: Human tonsil.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS containing 0.09 % sodium azide. Sourced in TCS.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for TRF1

Target: TRF1 (TERF1) (Telomeric Repeat Binding Factor (NIMA-Interacting) 1 (TERF1))

Alternative Name: TRF1 / TERF1

Background: Name/Gene ID: TERF1

Synonyms: TERF1, HTRF1-AS, NIMA-interacting protein 2, PIN2, Telomeric protein Pin2/TRF1, TRBF1, TTAGGG repeat-binding factor 1, T-TRF1, TRF, TRF1

Gene ID: 7013

Pathways: Cell Division Cycle, Telomere Maintenance, Hormone Transport, Chromatin Binding, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity