p300 antibody (pSer1834) (Cy3)

Catalog No. ABIN800616

This anti-p300 antibody is a Rabbit Polyclonal antibody detecting p300 in WB, IF (cc) and IF (p). Suitable for Human, Mouse and Zebrafish (Danio rerio).

Quick Overview for p300 antibody (pSer1834) (Cy3) (ABIN800616)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p300 antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-p300 Antibody (Cy3)

Binding Specificity: pSer1834

Cross-Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Predicted Reactivity: Rat,Dog,Cow,Pig,Horse,Chicken,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Ep300 around the phosphorylation site of Ser1834

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: Ep300

Background:

Synonyms: CREBBP/EP300 inhibitory protein 1, Cyclic AMP responsive enhancer binding protein, E1A associated protein p300, E1A binding protein p300, EC 2.3.1.48, EP300, EP300: E1A binding protein p300, RB and P300 binding protein EID 1, Histone acetyltransferase p300, p300 HAT, Retinoblastoma protein associated protein.

Background: KAT3B/p300 is a transcriptional adapter protein (300 kDa)which is characterized by three cysteine and histidine rich regions and its C-terminus specifically binds the adenovirus E1A protein. KAT3B and associated proteins are components of TATA-binding protein (TBP)complexes. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265 kDa nuclear protein designated KAT3A/CBP(for CREB-binding protein). KAT3B and KAT3A are homologous to each other.

Gene ID: 2033

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size