E-cadherin antibody (PE)

Catalog No. ABIN807921

Product Details anti-E-cadherin Antibody (PE)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E-cadherin antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody 67A4 recognizes an extracellular epitope of CD324 / E-cadherin, an approximately 100 kDa epithelial cell adhesion molecule, whose detection is important for determination of invasive potential of epithelial neoplasms.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: T-47D cells

Clone: 67A4

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-E-cadherin antibody (PE) (ABIN807921)

Lin, Liao, Lee, Hung, Sayion, Chen, Kang, Huang, Cherng: "Molecular events associated with epithelial to mesenchymal transition of nasopharyngeal carcinoma cells in the absence of Epstein-Barr virus genome." in: Journal of biomedical science, Vol. 16, pp. 105, (2009) (PubMed).

Furio, Guezennec, Ducarre, Guesnet, Peguet-Navarro: "Differential effects of allergens and irritants on early differentiating monocyte-derived dendritic cells." in: European journal of dermatology : EJD, Vol. 18, Issue 2, pp. 141-7, (2008) (PubMed).

Caberg, Hubert, Begon, Herfs, Roncarati, Boniver, Delvenne: "Silencing of E7 oncogene restores functional E-cadherin expression in human papillomavirus 16-transformed keratinocytes." in: Carcinogenesis, Vol. 29, Issue 7, pp. 1441-7, (2008) (PubMed).

Robertson, Ali, McDonnell, Burt, Kirby: "Chronic renal allograft dysfunction: the role of T cell-mediated tubular epithelial to mesenchymal cell transition." in: Journal of the American Society of Nephrology : JASN, Vol. 15, Issue 2, pp. 390-7, (2004) (PubMed).

Novak, Kraft, Haberstok, Geiger, Allam, Bieber: "A reducing microenvironment leads to the generation of FcepsilonRIhigh inflammatory dendritic epidermal cells (IDEC)." in: The Journal of investigative dermatology, Vol. 119, Issue 4, pp. 842-9, (2002) (PubMed).

Kutlesa, Wessels, Speiser, Steiert, Müller, Klein: "E-cadherin-mediated interactions of thymic epithelial cells with CD103+ thymocytes lead to enhanced thymocyte cell proliferation." in: Journal of cell science, Vol. 115, Issue Pt 23, pp. 4505-15, (2002) (PubMed).

Servet-Delprat, Vidalain, Bausinger, Manié, Le Deist, Azocar, Hanau, Fischer, Rabourdin-Combe: "Measles virus induces abnormal differentiation of CD40 ligand-activated human dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 4, pp. 1753-60, (2000) (PubMed).

Armeanu, Bühring, Reuss-Borst, Müller, Klein: "E-cadherin is functionally involved in the maturation of the erythroid lineage." in: The Journal of cell biology, Vol. 131, Issue 1, pp. 243-9, (1995) (PubMed).

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: CD324 / E-Cadherin (CDH1 Products)

Background: Cadherin 1,CD324 / E-cadherin is an epithelial cell surface molecule, which provides calcium-dependent homophilic interactions with E-cadherin of another cell. These intaractions take part in morphogenetic programs controlling the maintenance of the structural and functional integrity of epithelia and affect invasive potential of epithelial neoplasms. CD324 / E-cadherin is implicated in cell growth and differentiation, cell recognition, and sorting during developmental morphogenesis, as well as in aggregation-dependent cell survival. CD324 / E-cadherin-mediated cell adhesion system is highly regulated from inside the cell by a number of intracellular signaling pathways.,Cadherin-1, E-cadherin, Uvomorulin, CAM 120/80, UVO, CDHE, ECAD, LCAM, BCDS1, Arc-1

Gene ID: 999

UniProt: P12830

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation