SYNGAP1 antibody (AA 1169-1183)

Catalog No. ABIN870656

The Goat Polyclonal anti-SYNGAP1 antibody has been validated for WB, ELISA, IF and IHC. It is suitable to detect SYNGAP1 in samples from Human and Mouse.

Quick Overview for SYNGAP1 antibody (AA 1169-1183) (ABIN870656)

Target: SYNGAP1 (Synaptic Ras GTPase Activating Protein 1 (SYNGAP1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SYNGAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-SYNGAP1 Antibody

Binding Specificity: AA 1169-1183

Purpose: SYNGAP1 (aa1169-1183)

Sequence: ESAHIEREEY KLKEY

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ESAHIEREEYKLKEY, from the internal region (near C Terminus) of the protein sequence according to NP_006763.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 4 μg/mL.

Western Blot: Approx 150 kDa band observed in Human Cerebral Cortex and in Mouse Brain lysates (calculated MW of 148 kDa according to Human NP_006763.2 and Mouse XP_990642.3). Recommended concentration: 0.5-2 μg/mL. Primary incubation 1 hour at room tempera

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in Neuro-2a, A431 and U251 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SYNGAP1

Target: SYNGAP1 (Synaptic Ras GTPase Activating Protein 1 (SYNGAP1))

Alternative Name: SYNGAP1

Background: SYNGAP1, synaptic Ras GTPase activating protein 1, DKFZp761G1421, KIAA1938, MRD5, RASA1, RASA5, SYNGAP, OTTHUMP00000209046, neuronal RasGAP, ras GTPase-activating protein SynGAP, synaptic Ras GTPase activating protein 1 homolog, synaptic Ras GTPase activa

Molecular Weight: 148kDa

Gene ID: 8831, 240057, 192117

NCBI Accession: NP_006763

Pathways: Regulation of long-term Neuronal Synaptic Plasticity