APP antibody (N-Term)

Catalog No. ABIN870659

The Goat Polyclonal anti-APP antibody has been validated for WB, ELISA and IHC. It is suitable to detect APP in samples from Human and Mouse.

Quick Overview for APP antibody (N-Term) (ABIN870659)

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This APP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-APP Antibody

Binding Specificity: N-Term

Purpose: Amyloid beta peptide (mouse)

Sequence: DAEFGHDSGF EVRHQK

Specificity: This antibody is expected to recognize all reported isoforms of mAPP (NP_001185752.1, NP_031497.2, NP_001185753.1, NP_001185754.1, NP_001185755.1) and to recognize all lengths of the putative mouse Amyloid beta peptide.

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence DAEFGHDSGFEVRHQK-C, from the N Terminus of the protein sequence according to NP_001185752.1, NP_031497.2, NP_001185753.1, NP_001185754.1, NP_001185755.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5 μg/mL.

Western Blot: Approx 100 kDa band observed in Mouse Brain lysates (calculated MW of 86.7 kDa according to NP_001185752.1). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Alternative Name: App

Background: App, amyloid beta (A4) precursor protein, Abeta, Abpp, Adap, Ag, Cvap, E030013M08Rik, betaApp, alzheimer disease amyloid A4 protein homolog, amyloid beta A4 protein, amyloidogenic glycoprotein, appican, protease nexin II

Molecular Weight: 86.7kDa

Gene ID: 11820, 54226

NCBI Accession: NP_001185752, NP_031497, NP_001185753, NP_001185754, NP_001185755

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour