MTHFR antibody (AA 369-383)

Catalog No. ABIN870660

Product Details anti-MTHFR Antibody

Target: MTHFR (Methylenetetrahydrofolate Reductase (NAD(P)H) (MTHFR))

Binding Specificity: AA 369-383

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This MTHFR antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: MTHFR (aa369-383)

Sequence: SRPKSYIYRT QEWDE

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-SRPKSYIYRTQEWDE, from the internal region of the protein sequence according to NP_005948.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 74.6 kDa according to Human NP_005948.3 and Mouse NP_034970.2). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MTHFR

Target: MTHFR (Methylenetetrahydrofolate Reductase (NAD(P)H) (MTHFR))

Alternative Name: MTHFR (MTHFR Products)

Background: MTHFR, methylenetetrahydrofolate reductase (NAD(P)H), 5,10-methylenetetrahydrofolate reductase (NADPH), OTTHUMP00000002367, OTTHUMP00000002373, methylenetetrahydrofolate reductase

Molecular Weight: 74.6kDa

Gene ID: 4524, 17769, 362657

NCBI Accession: NP_005948

Pathways: Methionine Biosynthetic Process