NPR3 antibody (AA 520-533)

Catalog No. ABIN870670

This Goat Polyclonal antibody specifically detects NPR3 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for NPR3 antibody (AA 520-533) (ABIN870670)

Target: NPR3 (Atrial Natriuretic Peptide Receptor 3 (NPR3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NPR3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-NPR3 Antibody

Binding Specificity: AA 520-533

Purpose: NPR3 (aa520-533)

Sequence: HRELREDSIR SHFS

Specificity: The immunizing peptide represents the cytoplasmic part of the protein.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HRELREDSIRSHFS, from the C Terminus of the protein sequence according to NP_000899.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows staining of brush borders in select PCT. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Prostate. Recommended concentration: 10 μg/mL.

Western Blot: Approx 52 kDa band observed in Human Placenta lysates (calculated MW of 59.8 kDa according to NP_000899.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NPR3

Target: NPR3 (Atrial Natriuretic Peptide Receptor 3 (NPR3))

Alternative Name: NPR3

Background: NPR3, natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C), ANPRC, GUCY2B, NPRC, ANP-C, ANPR-C, NPR-C, OTTHUMP00000197048, OTTHUMP00000220980, atrial natriuretic peptide clearance receptor, atrial natriuretic peptide re

Molecular Weight: 59.8kDa

Gene ID: 4883, 18162, 25339

NCBI Accession: NP_000899

Pathways: cAMP Metabolic Process