NEK1 antibody (AA 301-400)

Catalog No. ABIN872417

The Rabbit Polyclonal anti-NEK1 antibody has been validated for ELISA, WB, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect NEK1 in samples from Mouse and Rat. There is 1 publication available.

Quick Overview for NEK1 antibody (AA 301-400) (ABIN872417)

Target: NEK1 (NIMA-Related Kinase 1 (NEK1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NEK1 Antibody

Binding Specificity: AA 301-400

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Sheep,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NEK1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-NEK1 antibody (ABIN872417)

Yeo, Becherel, Luff, Graham, Richard, Lavin: "Senataxin controls meiotic silencing through ATR activation and chromatin remodeling." in: Cell discovery, Vol. 1, pp. 15025, (2016) (PubMed).

Target Details for NEK1

Target: NEK1 (NIMA-Related Kinase 1 (NEK1))

Alternative Name: NEK1

Background:

Synonyms: SRPS2, SRTD6, SRPS2A, NY-REN-55, Serine/threonine-protein kinase Nek1, Never in mitosis A-related kinase 1, NimA-related protein kinase 1, Renal carcinoma antigen NY-REN-55, NEK1, KIAA1901

Background: Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity. Implicated in the control of meiosis (By similarity). Involved in cilium assembly. In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death.

Gene ID: 4750

UniProt: Q96PY6