NUF2 antibody (AA 201-300)

Catalog No. ABIN872897

The Rabbit Polyclonal anti-NUF2 antibody has been validated for ELISA, FACS, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect NUF2 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for NUF2 antibody (AA 201-300) (ABIN872897)

Target: NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUF2 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NUF2 Antibody

Binding Specificity: AA 201-300

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CDCA1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-NUF2 antibody (ABIN872897)

Tokuzumi, Fukushima, Miyashita, Nakahara, Kubo, Yamashita, Harada, Nakamura, Kajihara, Jinnin, Ihn: "Cell division cycle-associated protein 1 as a new melanoma-associated antigen." in: The Journal of dermatology, Vol. 43, Issue 12, pp. 1399-1405, (2016) (PubMed).

Target Details for NUF2

Target: NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))

Alternative Name: CDCA1

Background:

Synonyms: CDCA1, CT106, NUF2R, Kinetochore protein Nuf2, hNuf2, hNuf2R, hsNuf2, Cell division cycle-associated protein 1, NUF2

Background: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.

Gene ID: 83540

UniProt: Q9BZD4

Pathways: Maintenance of Protein Location