PUS10 antibody (AA 101-200)

Catalog No. ABIN873013

This Rabbit Polyclonal antibody specifically detects PUS10 in WB, ELISA, IF (cc), IF (p), IHC (fro) and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PUS10 antibody (AA 101-200) (ABIN873013)

Target: PUS10 (Pseudouridylate Synthase 10 (PUS10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUS10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PUS10 Antibody

Binding Specificity: AA 101-200

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PUS10/CCDC139

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PUS10

Target: PUS10 (Pseudouridylate Synthase 10 (PUS10))

Alternative Name: CCDC139

Background:

Synonyms: CCDC139, Coiled coil domain containing protein 139, DOBI, FLJ32312, MGC126729, Putative tRNA pseudouridine synthase Pus10, tRNA pseudouridine 55 synthase, tRNA uridine isomerase, PUS10_HUMAN.

Background: Pseudouridination is the isomerization of uridine to pseudouridine. It is the most common posttranscriptional nucleotide modification found in RNA. It is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones which facilitate the correct folding and assembly of tRNAs.