PERK antibody (pThr980) (AbBy Fluor® 647)

Catalog No. ABIN878623

This Rabbit Polyclonal antibody specifically detects PERK in WB, FACS, IF (cc) and IF (p). It exhibits reactivity toward Human, Mouse, Rat, Dog and Pigand has been mentioned in 1 publication.

Quick Overview for PERK antibody (pThr980) (AbBy Fluor® 647) (ABIN878623)

Target: PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))

Reactivity: Human, Mouse, Rat, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PERK antibody is conjugated to AbBy Fluor® 647

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-PERK Antibody (AbBy Fluor® 647)

Binding Specificity: pThr980

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Predicted Reactivity: Cow,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from mouse PERK around the phosphorylation site of Thr980

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-PERK antibody (AbBy Fluor® 647) (ABIN878623)

Chen, Holstein, Aime, Bollo, Lechleiter: "Calcineurin β protects brain after injury by activating the unfolded protein response." in: Neurobiology of disease, Vol. 94, pp. 139-56, (2016) (PubMed).

Target Details for PERK

Target: PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))

Alternative Name: PERK

Background:

Synonyms: Pek, Perk, AI427929, Eukaryotic translation initiation factor 2-alpha kinase 3, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, Eif2ak3

Background: Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.

Gene ID: 13666

UniProt: Q9Z2B5

Pathways: Hormone Transport, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded Protein Response