C-JUN antibody (pTyr170) (AbBy Fluor® 488)

Catalog No. ABIN879107

The Rabbit Polyclonal anti-C-JUN antibody has been validated for WB, FACS and IF (cc). It is suitable to detect C-JUN in samples from Human.

Quick Overview for C-JUN antibody (pTyr170) (AbBy Fluor® 488) (ABIN879107)

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C-JUN antibody is conjugated to AbBy Fluor® 488

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-C-JUN Antibody (AbBy Fluor® 488)

Binding Specificity: pTyr170

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Tyr170

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for C-JUN

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Alternative Name: c-Jun

Background:

Synonyms: c-Jun phospho Y170, p-c-Jun phospho Y170, c-Jun Tyr170, Jun oncogene, JUN, Activator Protein 1, AP 1, AP1, AP-1, Enhancer Binding Protein AP1, Jun Activation Domain Binding Protein, JUN protein, JUNC, p39, Proto oncogene cJun, Transcription Factor AP1, V jun avian sarcoma virus 17 oncogene homolog, vJun Avian Sarcoma Virus 17 Oncogene Homolog, JUN_HUMAN, Transcription factor AP-1, Activator 1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog.

Background: The human protooncogene JUN is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. cJun (previously known as the Fos binding protein p39) and c Fos form a complex in the nucleus. AP 1 (activating protein 1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP 1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16. Fos and Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. JUN has been mapped to 1p32 to p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Gene ID: 3725

Pathways: MAPK Signaling, RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins