Cortactin antibody (pThr466) (AbBy Fluor® 350)

Catalog No. ABIN879745

This Rabbit Polyclonal antibody specifically detects Cortactin in WB, IF (cc) and IF (p). It exhibits reactivity toward Human.

Quick Overview for Cortactin antibody (pThr466) (AbBy Fluor® 350) (ABIN879745)

Target: Cortactin (CTTN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cortactin antibody is conjugated to AbBy Fluor® 350

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Cortactin Antibody (AbBy Fluor® 350)

Binding Specificity: pThr466

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Cortactin around the phosphorylation site of Tyr466(YAp-TEA)

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Cortactin

Target: Cortactin (CTTN)

Alternative Name: Cortactin

Background:

Synonyms: Amplaxin, CTTN, EMS 1, EMS1, Mammary tumor and squamous cell carcinoma associated, Oncogene EMS1, p80/85 src substrate, Src substrate cortactin, SRC8_HUMAN.

Background: Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.

Gene ID: 2017

Pathways: MAPK Signaling