CD4 antibody (AA 385-457) (AbBy Fluor® 555)

Catalog No. ABIN889094

The Rabbit Polyclonal anti-CD4 antibody has been validated for FACS, WB, IF (cc) and IF (p). It is suitable to detect CD4 in samples from Human, Mouse, Rat, Chicken and Pig.

Quick Overview for CD4 antibody (AA 385-457) (AbBy Fluor® 555) (ABIN889094)

Target: CD4

Reactivity: Human, Mouse, Rat, Chicken, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD4 antibody is conjugated to AbBy Fluor® 555

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-CD4 Antibody (AbBy Fluor® 555)

Binding Specificity: AA 385-457

Cross-Reactivity: Chicken, Human, Mouse, Pig, Rat

Predicted Reactivity: Dog,Cow,Sheep,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Human CD4 (cytoplasmic domain)

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background:

Synonyms: T-cell surface glycoprotein CD4, Cd4, T-cell surface antigen T4/Leu-3, W3/25 antigen

Background: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.

Gene ID: 24932

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events