Cyclin D1 antibody (AA 61-110) (AbBy Fluor® 555)

Catalog No. ABIN892917

This anti-Cyclin D1 antibody is a Rabbit Polyclonal antibody detecting Cyclin D1 in WB, FACS, IF (cc) and IF (p). Suitable for Human, Mouse, Rat, Cow, Rabbit and Sheep. This Primary Antibody has been cited in 1 publication.

Quick Overview for Cyclin D1 antibody (AA 61-110) (AbBy Fluor® 555) (ABIN892917)

Target: Cyclin D1 (CCND1)

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D1 antibody is conjugated to AbBy Fluor® 555

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Cyclin D1 Antibody (AbBy Fluor® 555)

Binding Specificity: AA 61-110

Cross-Reactivity: Cow, Human, Mouse, Rabbit, Rat, Sheep

Predicted Reactivity: Dog

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse Cyclin D1

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Cyclin D1 antibody (AbBy Fluor® 555) (ABIN892917)

Souza, Silva, Calloway, Kucuk, Rossi, McLemore: "Genistein protects hematopoietic stem cells against G-CSF-induced DNA damage." in: Cancer prevention research (Philadelphia, Pa.), Vol. 7, Issue 5, pp. 534-44, (2014) (PubMed).

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: Cyclin D1

Background:

Synonyms: cD1, CycD1, Cyl-1, PRAD1, bcl-1, AI32739, G1/S-specific cyclin-D1, Ccnd1

Background: Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity).

Gene ID: 12443

UniProt: P25322

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling