FAM21C antibody (AA 1201-1318) (HRP)

Catalog No. ABIN896483

The Rabbit Polyclonal anti-FAM21C antibody is suitable to detect FAM21C in samples from Rat. It has been validated for ELISA, IHC (fro) and IHC (p).

Quick Overview for FAM21C antibody (AA 1201-1318) (HRP) (ABIN896483)

Target: FAM21C (Family with Sequence Similarity 21, Member C (FAM21C))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAM21C antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FAM21C Antibody (HRP)

Binding Specificity: AA 1201-1318

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Cow,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FAM21C

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FAM21C

Target: FAM21C (Family with Sequence Similarity 21, Member C (FAM21C))

Alternative Name: FAM21C

Background:

Synonyms: FAM21A, Family with sequence similarity 21 member C, Hypothetical protein LOC253725, KIAA0592, Protein FAM21C, FA21C_HUMAN.

Background: Playing a essential role in the fission of tubules that serve as transport intermediates during endosome sorting, the WASH complex is present at the surface of endosomes and functions to recruit and activate the Arp2/3 complex for induction of actin polymerization. FAM21, Putative WASH complex subunit FAM21, is a component of the WASH complex. Having undergone evolutionary duplication, four highly homologous family members exist including FAM21A, FAM21B, FAM21C and FAM21D. FAM21 links the WASH complex to endosomes and is required for WASM-dependent retromer-mediated sorting. Also, by directly interacting with CapZ, FAM21 inhibits its anti-capping activity, thereby regulating actin dynamics.

Gene ID: 253725