TLR5 antibody (C-Term)

Catalog No. ABIN920613

The Rabbit Polyclonal anti-TLR5 antibody (ABIN920613) specifically detects TLR5 in WB and ICC. The antibody is reactive with Human and Mouse samples.

Quick Overview for TLR5 antibody (C-Term) (ABIN920613)

Target: TLR5 (Toll-Like Receptor 5 (TLR5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR5 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-TLR5 Antibody

Binding Specificity: C-Term

Characteristics: Positive Control: Found in the membrane, TLR5 is highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.

Purification: Antigen Immunoaffinity purified

Immunogen: Rabbit polyclonal TLR5 antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of TLR5.

Isotype: IgG

Application Details

Application Notes: Western blot: use at 1-2 µg/mL. Immunocytochemistry: use at 10 µg/mL

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.02% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for TLR5

Target: TLR5 (Toll-Like Receptor 5 (TLR5))

Background: Toll-like receptors (TRLs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR5 recognizes flagellin from both Gram-positive and Gram-negative bacteria and will cause the activation of NF-B, leading to the activation of TNF-alpha and other cytokines. A common TLR5 stop codon polymorphism that disrupts TLR5 signaling is associated with susceptibility to Legionnaires' disease and demonstrates the importance of TLR5 in the innate immune response. Synonyms:

Molecular Weight: 97.8 kDa

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades