His Tag antibody

Catalog No. ABIN920801

The Chicken Polyclonal anti-His Tag antibody has been validated for WB and ELISA. It is suitable to detect His Tag in samples from .

Quick Overview for His Tag antibody (ABIN920801)

Target: His Tag

Reactivity: Please inquire

Host: Chicken

Clonality: Polyclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-His Tag Antibody

Sequence: HHHHHH

Purification: Affinity purified

Application Details

Application Notes: Western blot: Use at 1:1,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS pH 7.2 and 0.1% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for His Tag

Target: His Tag

Alternative Name: Hexahistidine-Tag

Target Type: Tag

Background: Chickens were immunized with HHHHHH peptide conjugated to KLH. After multiple immunizations in Freund's adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity- purified using the peptide immobilized on a solid support. Specification: Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH- BSA). A 1:8,000 dilution of the antibody gave an OD = 1.0 in a 15 minute reaction using HRP- conjugated goat anti-chicken IgY (IgG) at 1:10,000 at TMB as the substrate. Appropriate specificity controls were run.