Cullin 2 antibody (Middle Region)

Catalog No. ABIN926903

The Rabbit Polyclonal anti-Cullin 2 antibody has been validated for WB. It is suitable to detect Cullin 2 in samples from Human, Mouse, Rat, Dog, Cow and Xenopus laevis.

Quick Overview for Cullin 2 antibody (Middle Region) (ABIN926903)

Target: Cullin 2 (CUL2)

Reactivity: Human, Mouse, Rat, Dog, Cow, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Cullin 2 Antibody

Binding Specificity: Middle Region

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Frog

Purification: Purified

Immunogen: CUL2 antibody was raised in rabbit using the middle region of CUL2 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

CUL2 Blocking Peptide, , is also available for use as a blocking control in assays to test for specificity of this CUL2 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for Cullin 2

Target: Cullin 2 (CUL2)

Alternative Name: CUL2

Background: CUL2 is a core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. This protein may serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 By similarity. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). Synonyms: Polyclonal CUL2 antibody, Anti-CUL2 antibody, cullin 2 antibody, MGC131970 antibody.

Pathways: M Phase, Asymmetric Protein Localization, SARS-CoV-2 Protein Interactome