Cyclin D1 antibody (Middle Region)

Catalog No. ABIN926904

This anti-Cyclin D1 antibody is a Rabbit Polyclonal antibody detecting Cyclin D1 in WB. Suitable for Human, Mouse, Rat, Cow, Chicken, Dog, Zebrafish (Danio rerio) and Xenopus laevis.

Quick Overview for Cyclin D1 antibody (Middle Region) (ABIN926904)

Target: Cyclin D1 (CCND1)

Reactivity: Human, Mouse, Rat, Cow, Chicken, Dog, Zebrafish (Danio rerio), Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Cyclin D1 Antibody

Binding Specificity: Middle Region

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Zebrafish (Brachydanio rerio), Frog, Chicken

Purification: Purified

Immunogen: CCND1 antibody was raised in rabbit using the middle region of CCND1 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

CCND1 Blocking Peptide, , is also available for use as a blocking control in assays to test for specificity of this CCND1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: CCND1

Background: CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Synonyms: Polyclonal CCND1 antibody, Anti-CCND1 antibody, cyclin D1 antibody, BCL1 antibody, D11S287E antibody, PRAD1 antibody, U21B31 antibody.

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling