CDC25B antibody (N-Term)

Catalog No. ABIN926908

This Rabbit Polyclonal antibody specifically detects CDC25B in WB and IHC. It exhibits reactivity toward Human, Dog and Pig.

Quick Overview for CDC25B antibody (N-Term) (ABIN926908)

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Reactivity: Human, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CDC25B Antibody

Binding Specificity: N-Term

Cross-Reactivity: Pig (Porcine), Dog (Canine)

Purification: Purified

Immunogen: CDC25 B antibody was raised in rabbit using the N terminal of CDC25 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

CDC25B Blocking Peptide, , is also available for use as a blocking control in assays to test for specificity of this CDC25B antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for CDC25B

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Alternative Name: CDC25B

Background: CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Synonyms: Polyclonal CDC25B antibody, Anti-CDC25B antibody, cell division cycle 25 homolog B, S. pombe antibody.

Pathways: Cell Division Cycle, M Phase, Autophagy