PML antibody (C-Term)

Catalog No. ABIN927422

The Rabbit Polyclonal anti-PML antibody has been validated for WB. It is suitable to detect PML in samples from Human, Mouse and Rat.

Quick Overview for PML antibody (C-Term) (ABIN927422)

Target: PML (Promyelocytic Leukemia (PML))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PML antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PML Antibody

Binding Specificity: C-Term

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Purified

Immunogen: PML antibody was raised in rabbit using the C terminal of PML as the immunogen

Application Details

Application Notes: Optimal conditions should be determined by the investigator.

Comment:

PML Blocking Peptide, (ABIN937829), is also available for use as a blocking control in assays to test for specificity of this PML antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for PML

Target: PML (Promyelocytic Leukemia (PML))

Alternative Name: PML

Background: PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. PML localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions, all variants encode the same N-terminus. Synonyms: Polyclonal PML antibody, Anti-PML antibody, promyelocytic leukemia antibody, MYL antibody, PP8675 antibody, RNF71 antibody, TRIM19 antibody.

Pathways: p53 Signaling, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus