KEAP1 antibody (C-Term)

Catalog No. ABIN927752

The Rabbit Polyclonal anti-KEAP1 antibody has been validated for WB and IHC. It is suitable to detect KEAP1 in samples from Human, Mouse, Rat, Pig, Cow and Xenopus laevis.

Quick Overview for KEAP1 antibody (C-Term) (ABIN927752)

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reactivity: Human, Mouse, Rat, Pig, Cow, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KEAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-KEAP1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Cow (Bovine), Pig (Porcine), Frog

Purification: Purified

Immunogen: KEAP1 antibody was raised in rabbit using the C terminal of KEAP1 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

KEAP1 Blocking Peptide, (ABIN939247), is also available for use as a blocking control in assays to test for specificity of this KEAP1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Alternative Name: KEAP1

Background: KEAP1 contains KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests. Synonyms: Polyclonal KEAP1 antibody, Anti-KEAP1 antibody, kelch-like ECH-associated protein 1 antibody.

Pathways: Maintenance of Protein Location