UBA3 antibody (N-Term)

Catalog No. ABIN928369

This Rabbit Polyclonal antibody specifically detects UBA3 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Chicken and Xenopus laevis.

Quick Overview for UBA3 antibody (N-Term) (ABIN928369)

Target: UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBA3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBA3 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Cow (Bovine), Dog (Canine), Chicken, Frog, Zebrafish (Brachydanio rerio)

Purification: Purified

Immunogen: UBA3 antibody was raised in rabbit using the N terminal of UBA3 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

UBA3 Blocking Peptide, (ABIN937832), is also available for use as a blocking control in assays to test for specificity of this UBA3 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for UBA3

Target: UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))

Alternative Name: UBA3

Background: UBA3 is a catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. UBA3 down-regulates steroid receptor activity. UBA3 is necessary for cell cycle progression.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. Synonyms: Polyclonal UBA3 antibody, Anti-UBA3 antibody, ubiquitin-like modifier activating enzyme 3 antibody, DKFZp566J164 antibody, MGC22384 antibody, UBE1C antibody, hUBA3 antibody.