CISH antibody (N-Term)

Catalog No. ABIN928660

This Rabbit Polyclonal antibody specifically detects CISH in WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for CISH antibody (N-Term) (ABIN928660)

Target: CISH (Cytokine Inducible SH2-Containing Protein (CISH))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CISH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CISH Antibody

Binding Specificity: N-Term

Cross-Reactivity: Mouse (Murine)

Purification: Purified

Immunogen: Cish antibody was raised in rabbit using the N terminal of Cish as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

Cish Blocking Peptide, (ABIN5612894), is also available for use as a blocking control in assays to test for specificity of this Cish antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for CISH

Target: CISH (Cytokine Inducible SH2-Containing Protein (CISH))

Alternative Name: Cish

Background: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Synonyms: Polyclonal Cish antibody, Anti-Cish antibody, cytokine inducible SH2-containing protein antibody, AI385595 antibody, CIS1 antibody, Cis antibody, F17 antibody, F23 antibody.

Pathways: JAK-STAT Signaling, Response to Growth Hormone Stimulus