E2F5 antibody

Catalog No. ABIN931153

This Mouse Monoclonal antibody specifically detects E2F5 in DB and ICC. It exhibits reactivity toward Human.

Quick Overview for E2F5 antibody (ABIN931153)

Target: E2F5

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E2F5 antibody is un-conjugated

Application: Dot Blot (DB), Immunocytochemistry (ICC)

Clone: 220D1a

Product Details anti-E2F5 Antibody

Cross-Reactivity: Human

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: E2 F5 antibody was raised in mouse using recombinant Human Transcription Factor E2 -5

Isotype: IgG1

Application Details

Application Notes: ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: E2 F5 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for E2F5

Target: E2F5

Alternative Name: E2F5

Background: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Synonyms: Monoclonal E2F5 antibody, Anti-E2F5 antibody, E2F transcription factor 5 p130-binding antibody.