Ki-67 antibody (AA 3118-3256)

Catalog No. ABIN931472

The Mouse Monoclonal anti-Ki-67 antibody is suitable to detect Ki-67 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for Ki-67 antibody (AA 3118-3256) (ABIN931472)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ki-67 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 9C12B2

Product Details anti-Ki-67 Antibody

Binding Specificity: AA 3118-3256

Immunogen: KI67 antibody was raised in Mouse using a purified recombinant fragment of KI167(aa3118-3256) expressed in E. coli as the immunogen.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:10,000, WB: 1:500-1:2000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Store at -20 °C for long term storage.

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: KI67

Background: KI67 is a nuclear protein, which is expressed in proliferating cells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. Synonyms: Monoclonal KI67 antibody, Anti-KI67 antibody, KI-67 antibody, KI 67 antibody.

Pathways: Glycosaminoglycan Metabolic Process