TAF6 antibody

Catalog No. ABIN933107

The Mouse Monoclonal anti-TAF6 antibody is suitable to detect TAF6 in samples from Human. It has been validated for WB, IP, ICC and DB.

Quick Overview for TAF6 antibody (ABIN933107)

Target: TAF6 (TAF6 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 80kDa (TAF6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TAF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Dot Blot (DB)

Clone: 585D4a

Product Details anti-TAF6 Antibody

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: TAF6 antibody was raised in mouse using recombinant Human Taf6 Rna Polymerase Ii, Tata Box Binding Protein (Tbp)-Associated Factor, 80 Kda (Taf6)

Isotype: IgG1

Application Details

Application Notes: WB: 0.2-2 µg/mL, IP: 100-500 µg/sample, ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: TAF6 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for TAF6

Target: TAF6 (TAF6 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 80kDa (TAF6))

Alternative Name: TAF6

Background: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Synonyms: Monoclonal TAF6 antibody, Anti-TAF6 antibody, Transcription initiation factor TFIID subunit 6 antibody, TAF2E antibody, TAFII70 antibody, TAFII80 antibody, TAFII85 antibody, MGC:8964 antibody, DKFZp781E21155 antibody.