PSMC5 antibody

Catalog No. ABIN933121

This Mouse Monoclonal antibody specifically detects PSMC5 in WB, ICC and DB. It exhibits reactivity toward Human.

Quick Overview for PSMC5 antibody (ABIN933121)

Target: PSMC5 (Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 5 (PSMC5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PSMC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Dot Blot (DB)

Clone: 2837C4a

Product Details anti-PSMC5 Antibody

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: PSMC5 antibody was raised in mouse using recombinant Human Proteasome (Prosome, Macropain) 26 S Subunit, Atpase, 5 (Psmc5)

Isotype: IgG1

Application Details

Application Notes: WB: 0.2-2 µg/mL, ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: PSMC5 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for PSMC5

Target: PSMC5 (Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 5 (PSMC5))

Alternative Name: PSMC5

Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a nonlysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. Synonyms: Monoclonal PSMC5 antibody, Anti-PSMC5 antibody, 26S protease regulatory subunit 8 antibody, S8 antibody, p45 antibody, SUG1 antibody, TBP10 antibody, TRIP1 antibody, p45/SUG antibody.

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway