DYKDDDDK Tag antibody

Catalog No. ABIN933978

The Mouse Monoclonal anti-DYKDDDDK Tag antibody (Clone FG4R) (ABIN933978) specifically detects DYKDDDDK Tag in WB, ELISA, IP and DB.

Quick Overview for DYKDDDDK Tag antibody (ABIN933978)

Target: DYKDDDDK Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This DYKDDDDK Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Clone: FG4R

Product Details anti-DYKDDDDK Tag Antibody

Sequence: DYKDDDD

Purification: Protein A affinity chromatography

Immunogen: FLAG antibody was raised in mouse using DYKDDDDK (FLAG) synthetic peptide conjugated to KLH as the immunogen.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:1000-3000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Alternative Name: DYKDDDD-Tag

Target Type: Tag

Background: DYKDDDDK tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A DYKDDDDK-Tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a DYKDDDDK-Tag to this protein allows one to follow the protein with an antibody against the DYKDDDDK sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Synonyms: Monoclonal DYKDDDDK antibody, Anti DYKDDDDK antibody, DYKDDDDK antibody, DYKDDDDK antibody, FG4R antibody, DYKDDDDK tag antibody.