COXII antibody

Catalog No. ABIN93555

This anti-COXII antibody is a Chicken Polyclonal antibody detecting COXII in WB. Suitable for Arabidopsis thaliana, Moss, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora and Wild Cabbage. This Primary Antibody has been cited in 4+ publications.

Quick Overview for COXII antibody (ABIN93555)

Target: COXII (Cytochrome Oxidase Subunit II (COXII))

Reactivity: Arabidopsis thaliana, Moss, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Wild Cabbage

Host: Chicken

Clonality: Polyclonal

Conjugate: This COXII antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-COXII Antibody

Specificity: Arabidopsis thaliana (leaf extract and isolated mitochondria).

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: eudicots, monocots

Characteristics: Expected / apparent Molecular Weight of the Antigene: 29.4 / 30 kDa (for Arabidopsis thaliana)

Purification: affinity purified

Immunogen: KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots includingArabidopsis thaliana AtmG00160, monocots including Oryza sativa P04373 and Physcomitrella patens Q1XGA9

Isotype: IgY

Application Details

Application Notes: 1:1000 with standard ECL (WB)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS pH 8.0+ 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Make aliquots to avoid repreated freeze-thaw cycles and working with a stock.

Storage: 4 °C

Storage Comment: store at 4°C, make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

References for anti-COXII antibody (ABIN93555)

Yoshida, Watanabe, Hachiya, Tholen, Shibata, Terashima, Noguchi: "Distinct responses of the mitochondrial respiratory chain to long- and short-term high-light environments in Arabidopsis thaliana." in: Plant, cell & environment, (2011) (PubMed).

Moellering, Benning: "Phosphate regulation of lipid biosynthesis in Arabidopsis is independent of the mitochondrial outer membrane DGS1 complex." in: Plant physiology, Vol. 152, Issue 4, pp. 1951-9, (2010) (PubMed).

Grant, Miller, Watling, Robinson: "Synchronicity of thermogenic activity, alternative pathway respiratory flux, AOX protein content, and carbohydrates in receptacle tissues of sacred lotus during floral development." in: Journal of experimental botany, Vol. 59, Issue 3, pp. 705-14, (2008) (PubMed).

Leroch, Neuhaus, Kirchberger, Zimmermann, Melzer, Gerhold, Tjaden: "Identification of a novel adenine nucleotide transporter in the endoplasmic reticulum of Arabidopsis." in: The Plant cell, Vol. 20, Issue 2, pp. 438-51, (2008) (PubMed).

Target Details for COXII

Target: COXII (Cytochrome Oxidase Subunit II (COXII))

Alternative Name: COXII

Background: AGI Code: ATMG00160
Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.

Molecular Weight: expected: 29.4 kDa, apparent: 30 kDa (for Arabidopsis thaliana)

UniProt: P04373, Q1XGA9