alpha Tubulin antibody

Catalog No. ABIN93894

This anti-alpha Tubulin antibody is a Mouse Monoclonal antibody detecting alpha Tubulin in WB, ELISA, IHC (p), IP and ICC. Suitable for Human, Mouse, Rat, Pig, Dog and Nicotiana tabacum. This Primary Antibody has been cited in 3+ publications.

Quick Overview for alpha Tubulin antibody (ABIN93894)

Target: alpha Tubulin (TUBA1)

Reactivity: Human, Mouse, Rat, Pig, Dog, Nicotiana tabacum

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: TU-16

Product Details anti-alpha Tubulin Antibody

Purpose: Anti-alpha-Tubulin Purified

Specificity: The antibody TU-16 reacts with alpha-tubulin of all tested species, under denaturing and non-denaturing conditions.

Cross-Reactivity (Details): Broad species reactivity

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Purity: > 95 % (by SDS-PAGE)

Immunogen: Porcine brain microtubule protein MTP-1.

Isotype: IgM

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL.
Immunoprecipitation: Reducing conditions.
Western blotting: Recommended dilution: 1-2 μg/mL. This antibody can be used for Western blotting, but its alternative TU-02 (11-447-C100) gives better signal in this application.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-alpha Tubulin antibody (ABIN93894)

Ji, Rath, Girton, Johansen, Johansen: "D-Hillarin, a novel W180-domain protein, affects cytokinesis through interaction with the septin family member Pnut." in: Journal of neurobiology, Vol. 64, Issue 2, pp. 157-69, (2005) (PubMed).

Qi, Rath, Wang, Xu, Ding, Zhang, Blacketer, Paddy, Girton, Johansen, Johansen: "Megator, an essential coiled-coil protein that localizes to the putative spindle matrix during mitosis in Drosophila." in: Molecular biology of the cell, Vol. 15, Issue 11, pp. 4854-65, (2004) (PubMed).

Dráberová, Dráber: "Novel monoclonal antibodies TU-08 and TU-16 specific for tubulin subunits." in: Folia biologica, Vol. 44, Issue 1, pp. 35-6, (2000) (PubMed).

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: alpha-Tubulin

Background: Tubulin alpha 1,The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.,TUBA

Gene ID: 7277

UniProt: Q71U36

Pathways: Microtubule Dynamics