beta-2 Microglobulin antibody

Catalog No. ABIN93900

Cited in 2+ publications. The Mouse Monoclonal anti-beta-2 Microglobulin antibody (Clone B2M-02) (ABIN93900) specifically detects beta-2 Microglobulin in WB, FACS, ELISA, ICC and IHC (p). The antibody is reactive with Human and Pig samples.

Quick Overview for beta-2 Microglobulin antibody (ABIN93900)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta-2 Microglobulin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: B2M-02

Product Details anti-beta-2 Microglobulin Antibody

Purpose: Anti-Hu beta2-Microglobulin Purified

Cross-Reactivity (Details): Human, Porcine

Characteristics: The mouse monoclonal antibody B2M-02 reacts with beta2-microglobulin (beta2M), an extracellular antigen associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Purified isolated human beta2-microglobulin.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Heat-mediated antigen retrieval, recommended dilution: 5 μg/mL, positive tissue: liver.
Western blotting: Recommended dilution: 2 μg/mL, non-reducing conditions preferred.
Flow cytometry: Recommended dilution: 1 μg/mL, positive control: PBL cell line, negative control: DAUDI human lymphoma cell line, erythrocytes.
ELISA: Working dilution should be determinated by investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-beta-2 Microglobulin antibody (ABIN93900)

Drbal, Angelisová, Hilgert, Cerný, Novák, Horejsí: "A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells." in: Blood, Vol. 98, Issue 5, pp. 1561-6, (2001) (PubMed).

Hilgert, Horejsí, Kristofová: "The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin." in: Folia biologica, Vol. 30, Issue 6, pp. 369-76, (1985) (PubMed).

Target Details for beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: beta2-Microglobulin

Background: Beta-2-microglobulin,Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.,B2M

Gene ID: 567

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process