MME antibody (Membrane Metallo-Endopeptidase)

Details for Product anti-MME Antibody No. ABIN93930
Antigen
Reactivity
Human
544
131
112
11
3
3
2
2
2
2
1
1
1
1
1
Host
Mouse
366
196
2
Clonality (Clone)
Monoclonal ()
Conjugate
This MME antibody is un-conjugated
56
43
41
29
8
7
7
6
5
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
283
235
177
129
89
80
64
35
31
21
13
13
13
6
6
2
1
1
1
Options
Immunogen NALM-6 human pre-B cell line
Clone MEM-78
Isotype IgG1
Specificity The antibody MEM-78 reacts with CD10 antigen (CALLA - Common acute lymphatic leukemia antigen), a 100 kDa type II integral membrane protein.
HLDA IV, WS Code B 506
HLDA V, WS Code B CD10.4
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD10 (MME Antibody Abstract)
Background CD10 (neutral endopeptidase –, NEP, common acute lymphocytic leukemia antigen –, CALLA, membrane metallo-endopeptidase –, MME, enkefalinase) is a 100- kDa cell surface zinc metalloprotease cleaving peptide bonds on the N-terminus of hydrophobic amino acids and inactivating multiple physiologically active peptids. CD10 is expressed on various normal cell types, including lymphoid precursor cells, germinal center B lymhocytes, and some epithelial cells, and its expression level serves as a marker for diagnostics of many carcinomas. CD10 is also a differentiation antigen for early B-lymphoid progenitors in the B-cell differentiation pathway and has a key role in regulation of growth, differentiation and signal transduction of many cellular systems.
Pathways RTK Signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Membrane Metallo-Endopeptidase (MME) antibody (ABIN93930) Surface staining of NALM-6 human pre-B cell leukemia cell line with anti-human CD10 (...
Product cited in: Angelisová, Drbal, Horejsí, Cerný: "Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase." in: Blood, Vol. 93, Issue 4, pp. 1437-9, 1999 (PubMed).