CD177 antibody (CD177 Molecule)

Details for Product anti-CD177 Antibody No. ABIN94001
Antigen
Reactivity
Human, Non-Human Primate
109
15
9
6
5
4
3
Host
Mouse
70
40
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD177 antibody is un-conjugated
13
12
11
4
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
71
58
42
26
15
12
4
1
1
1
1
Options
Immunogen Human granulocytes
Clone MEM-166
Isotype IgG1
Specificity The antibody MEM-166 reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.
HLDA VI, WS Code M M17
HLDA VI, WS Code BP 309
Cross-Reactivity (Details) Non-Human Primates, Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD177 (CD177 Antibody Abstract)
Background CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD177 Molecule (CD177) antibody (ABIN94001) Surface staining of human peripheral blood cells with anti-CD177 (MEM-166) FITC.