CD177 antibody

Catalog No. ABIN94001

The Mouse Monoclonal anti-CD177 antibody is suitable to detect CD177 in samples from Human and Non-Human Primate. It has been validated for WB, IP and FACS.

Quick Overview for CD177 antibody (ABIN94001)

Target: CD177 (CD177 Molecule (CD177))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD177 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: MEM-166

Product Details anti-CD177 Antibody

Purpose: Anti-Hu CD177 Purified

Cross-Reactivity (Details): Non-Human Primates, Human

Characteristics: The antibody MEM-166 reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human granulocytes

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1 μg/mL.
Western blotting: Non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD177

Target: CD177 (CD177 Molecule (CD177))

Alternative Name: CD177

Background: CD177 Molecule,CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).,NB1, PRV1, HNA2A

Gene ID: 57126

UniProt: Q8N6Q3