CD19 antibody (CD19 Molecule) (PE)

Details for Product anti-CD19 Antibody No. ABIN94016
Antigen
Reactivity
Human
792
354
74
63
20
20
8
7
5
4
4
4
4
4
4
4
4
2
2
Host
Mouse
637
222
182
12
Clonality (Clone)
Monoclonal ()
Conjugate
This CD19 antibody is conjugated to PE
109
100
78
72
28
22
16
16
15
14
14
13
12
9
9
8
8
8
8
8
7
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
753
205
170
144
129
121
59
54
44
40
22
15
9
8
5
4
4
2
2
1
1
1
1
1
Options
Immunogen Daudi human Burkitt lymphoma cell line
Clone LT19
Isotype IgG1
Specificity The antibody LT19 reacts with CD19 (B4), a 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells, it is lost on plasma cells.
HLDA 10
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD19 (CD19 Antibody Abstract)
Background CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD19 Molecule (CD19) antibody (PE) (ABIN94016) Surface staining of human peripheral blood cells with anti-human CD19 (LT19) APC.
Product cited in: Kayserova, Vcelakova, Stechova, Dudkova, Hromadkova, Sumnik, Kolouskova, Spisek, Sediva: "Decreased dendritic cell numbers but increased TLR9-mediated interferon-alpha production in first degree relatives of type 1 diabetes patients." in: Clinical immunology (Orlando, Fla.), Vol. 153, Issue 1, pp. 49-55, 2014 (PubMed).

Elias, Flo, Lopez, Zorzopulos, Montaner, Rodriguez: "Strong cytosine-guanosine-independent immunostimulation in humans and other primates by synthetic oligodeoxynucleotides with PyNTTTTGT motifs." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 7, pp. 3697-704, 2003 (PubMed).