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CD20 antibody (FITC)

MS4A1 Reactivity: Human FACS Host: Mouse Monoclonal LT20 FITC
Catalog No. ABIN94029
  • Target See all CD20 (MS4A1) Antibodies
    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
    Reactivity
    • 259
    • 57
    • 23
    • 18
    • 17
    • 16
    • 11
    • 7
    • 6
    • 5
    • 2
    • 2
    Human
    Host
    • 190
    • 96
    • 2
    • 2
    Mouse
    Clonality
    • 208
    • 80
    • 1
    Monoclonal
    Conjugate
    • 108
    • 21
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    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD20 antibody is conjugated to FITC
    Application
    • 218
    • 93
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    • 16
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    Flow Cytometry (FACS)
    Specificity
    The antibody LT20 reacts with an extracellular epitope of CD20 (Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.
    Cross-Reactivity (Details)
    Human
    Purification
    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogen
    Normal human lymphocytes from lymph node.
    Clone
    LT20
    Isotype
    IgG2a
    Top Product
    Discover our top product MS4A1 Primary Antibody
  • Application Notes
    Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
    Comment

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

    Restrictions
    For Research Use only
  • Reconstitution
    No reconstitution is necessary.
    Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Avoid prolonged exposure to light.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Všianská, Říhová, Varmužová, Suská, Kryukov, Mikulášová, Kupská, Penka, Pour, Adam, Hájek: "Analysis of B-cell subpopulations in monoclonal gammopathies." in: Clinical lymphoma, myeloma & leukemia, Vol. 15, Issue 4, pp. e61-71, (2015) (PubMed).

    Filatov, Krotov, Zgoda, Volkov: "Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry." in: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 21-33, (2007) (PubMed).

    Teeling, Mackus, Wiegman, van den Brakel, Beers, French, van Meerten, Ebeling, Vink, Slootstra, Parren, Glennie, van de Winkel: "The biological activity of human CD20 monoclonal antibodies is linked to unique epitopes on CD20." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 1, pp. 362-71, (2006) (PubMed).

    Chan, Hughes, French, Tutt, Walshe, Teeling, Glennie, Cragg: "CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts." in: Cancer research, Vol. 63, Issue 17, pp. 5480-9, (2003) (PubMed).

  • Target
    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
    Alternative Name
    CD20 (MS4A1 Products)
    Synonyms
    B1 antibody, Bp35 antibody, CD20 antibody, CVID5 antibody, LEU-16 antibody, MS4A2 antibody, S7 antibody, AA960661 antibody, Cd20 antibody, Ly-44 antibody, Ms4a2 antibody, MS4A1 antibody, bp35 antibody, cd20 antibody, ms4a2 antibody, leu-16 antibody, ms4a4c antibody, cd20-like antibody, membrane spanning 4-domains A1 antibody, membrane-spanning 4-domains, subfamily A, member 1 antibody, B-lymphocyte antigen CD20 antibody, MS4A1 antibody, Ms4a1 antibody, ms4a1 antibody, LOC101694281 antibody
    Background
    MS4A1,CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.,B1, S7, MS4A, Bp35, CVID5, LEU-16
    Gene ID
    931
    UniProt
    P11836
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