CD25 antibody (Interleukin 2 Receptor, alpha) (FITC)

Details for Product anti-IL2RA Antibody No. ABIN94046
Antigen
Reactivity
Human
469
298
101
17
15
6
6
5
5
4
4
3
1
Host
Mouse
415
240
151
4
3
3
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD25 antibody is conjugated to FITC
108
104
80
62
16
11
10
10
9
9
7
5
5
5
5
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
617
137
121
113
96
89
50
39
27
17
15
14
13
12
8
4
2
2
2
2
1
1
1
1
1
1
1
1
1
Options
Immunogen PHA-activated peripheral blood leucocytes
Clone MEM-181
Isotype IgG1
Specificity The antibody MEM-181 reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells), it is lost on resting B and T lymphocytes.
HLDA VI, WS Code NL N-L024
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 Molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (FITC) (ABIN94046) Surface staining of human PBMC with anti-human CD25 (MEM-181) FITC. The mononuclear c...
Product cited in: Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).