TERT antibody (AA 597-611)

Catalog No. ABIN940568

The Goat Polyclonal anti-TERT antibody has been validated for ELISA, FACS and IF. It is suitable to detect TERT in samples from Human.

Quick Overview for TERT antibody (AA 597-611) (ABIN940568)

Target: TERT (Telomerase Reverse Transcriptase (TERT))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TERT antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-TERT Antibody

Binding Specificity: AA 597-611

Purpose: TERT (aa597-611)

Sequence: QLRELSEAEV RQHRE

Specificity: This antibody is expected to recognize both reported isoforms (NP_937983.2, (NP_001180305.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QLRELSEAEVRQHRE, from the internal region of the protein sequence according to NP_937983.2, NP_001180305.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary testing showed a band at approx 125 kDa in lysates of cell lines A549 and CaCo-2 after 1 μg/mL antibody staining (calculated MW of 127 kDa according to NP_937983.2). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TERT

Target: TERT (Telomerase Reverse Transcriptase (TERT))

Alternative Name: TERT

Background: TERT, telomerase reverse transcriptase, EST2, TCS1, TP2, TRT, hEST2, telomerase catalytic subunit, telomerase-associated protein 2

Gene ID: 7015

NCBI Accession: NP_937983, NP_001180305

Pathways: Telomere Maintenance