CD41,CD61 antibody

Details for Product No. ABIN94110
Antigen
Reactivity
Cow (Bovine), Human
19
13
7
3
2
2
2
2
2
1
Host
Mouse
39
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
8
6
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP)
25
13
12
12
6
5
4
2
2
Options
Immunogen Bovine trombocytes.
Clone IVA30
Isotype IgG2
Specificity The antibody IVA30 reacts with CD41/CD61 complex (bovine), one of the earliest markers of the megakaryocytic lineage. CD41/CD61 complex acts as the receptor for fibrinogen, fibronectin, Von Willebrand factor, vitronectin, thrombin and mediates platelets aggregation. CD41/CD61 is expressed only by platelets and megakaryocytes, the complex may be absent or strongly reduced in Glanzmann's thrombasthenia (GT). This antibody crossreacts with human platelets.
Cross-Reactivity (Details) Human, Bovine
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Background CD41 is a transmembrane glycoprotein (integrin family) composed of two chains GPIIb alpha (heavy chain, 120 kDa) and GPIIb beta (light chain, 23 kDa). CD61 non-covalently associates with CD41.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: "Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995." in: Veterinary immunology and immunopathology, Vol. 52, Issue 4, pp. 213-468, 1997 (PubMed).

Mateo, Perez de la Lastra, Moreno, Dusinsky, Bilka, Simon, Horovska, Llanes: "Biochemical characterization of antigens detected with anti-platelet monoclonal antibodies." in: Veterinary immunology and immunopathology, Vol. 52, Issue 4, pp. 363-70, 1997 (PubMed).

Mateo, Pintado, Perez de la Lastra, Dusinsky, Simon, Naessens, Llanes: "Ruminant cluster CD41/CD61." in: Veterinary immunology and immunopathology, Vol. 52, Issue 4, pp. 251-3, 1997 (PubMed).

Naessens, Howard, Hopkins: "Nomenclature and characterization of leukocyte differentiation antigens in ruminants." in: Immunology today, Vol. 18, Issue 8, pp. 365-8, 1997 (PubMed).

Simon, Dusinsky, Horovska, Bilka, Hluchy: "Immunohistochemical reactivity of anti-platelet monoclonal antibodies." in: Veterinary immunology and immunopathology, Vol. 52, Issue 4, pp. 377-82, 1997 (PubMed).