CD44 antibody

Catalog No. ABIN94123

This Mouse Monoclonal antibody specifically detects CD44 in FACS, WB, ELISA and IP. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for CD44 antibody (ABIN94123)

Target: CD44

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: MEM-85

Product Details anti-CD44 Antibody

Purpose: Anti-Hu CD44 Purified

Specificity: The antibody MEM-85 reacts with an extracellular antigen of both cell surface-expressed and soluble form of CD44 antigen (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.), it is negative on platelets and hepatocytes.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Leukocytes of a patient suffering from LGL Type Leukaemia.

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: Recommended dilution: 2-6 μg/mL.
Western blotting: Recommended dilution: 2 μg/mL, 60 min on vertical incubator, positive control: Kg-1a human leukemia cell lysate, non-reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD44 antibody (ABIN94123)

Minamikawa, Nozu, Maeta, Yamamura, Nakanishi, Fujimura, Horinouchi, Nagano, Sakakibara, Nagase, Shima, Noda, Ninchoji, Kaito, Iijima: "The utility of urinary CD80 as a diagnostic marker in patients with renal diseases." in: Scientific reports, Vol. 8, Issue 1, pp. 17322, (2019) (PubMed).

Kolar, Mehta, Pelayo, Capra: "A novel human B cell subpopulation representing the initial germinal center population to express AID." in: Blood, Vol. 109, Issue 6, pp. 2545-52, (2007) (PubMed).

Bazil, Strominger: "Metalloprotease and serine protease are involved in cleavage of CD43, CD44, and CD16 from stimulated human granulocytes. Induction of cleavage of L-selectin via CD16." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1314-22, (1994) (PubMed).

Stefanová, Hilgert, Bazil, Kristofová, Horejsí: "Human leucocyte surface glycoprotein CDw44 and lymphocyte homing receptor are identical molecules." in: Immunogenetics, Vol. 29, Issue 6, pp. 402-4, (1989) (PubMed).

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: CD44 Molecule,CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.,PGP-I, HUTCH-I, ECMR-III, Hermes antigen, Hyaluronate receptor, Heparan sulfate proteoglycan, Epican, MC56, MIC4, INLU, LHR

Gene ID: 960

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling