CD44 antibody (CD44) (Biotin)

Details for Product anti-CD44 Antibody No. ABIN94124
Antigen
Reactivity
Human
792
341
169
48
43
42
35
31
29
26
16
14
11
10
10
6
5
4
2
1
1
1
1
1
1
1
1
1
Host
Mouse
638
222
198
12
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD44 antibody is conjugated to Biotin
142
88
73
43
11
11
9
8
8
8
8
6
6
6
6
6
6
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
680
405
317
223
178
174
172
114
40
40
33
33
16
16
14
13
8
6
5
3
3
3
3
1
1
1
1
Options
Immunogen Leukocytes of a patient suffering from LGL Type Leukaemia.
Clone MEM-85
Isotype IgG2b
Specificity The antibody MEM-85 reacts with both cell surface-expressed and soluble form of CD44 antigen (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.), it is negative on platelets and hepatocytes.
HLDA IV, WS Code NL 706
HLDA VI, WS Code AS Ref.15
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD44 (CD44 Antibody Abstract)
Background CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.
Pathways Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry.Suggested working dilution is 1:1000. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD44 (CD44) antibody (Biotin) (ABIN94124) Surface staining of human peripheral blood cells with anti-human CD44 (MEM-85) PE.
Product cited in: Kolar, Mehta, Pelayo, Capra: "A novel human B cell subpopulation representing the initial germinal center population to express AID." in: Blood, Vol. 109, Issue 6, pp. 2545-52, 2007 (PubMed).

Bazil, Strominger: "Metalloprotease and serine protease are involved in cleavage of CD43, CD44, and CD16 from stimulated human granulocytes. Induction of cleavage of L-selectin via CD16." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1314-22, 1994 (PubMed).

Stefanová, Hilgert, Bazil, Kristofová, Horejsí: "Human leucocyte surface glycoprotein CDw44 and lymphocyte homing receptor are identical molecules." in: Immunogenetics, Vol. 29, Issue 6, pp. 402-4, 1989 (PubMed).