CD45 antibody

Catalog No. ABIN94134

This anti-CD45 antibody is a Mouse Monoclonal antibody detecting CD45 in FACS, IP and ICC. Suitable for Cow. This Primary Antibody has been cited in 3+ publications.

Quick Overview for CD45 antibody (ABIN94134)

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: IVA103

Product Details anti-CD45 Antibody

Purpose: Anti-Bov CD45R Purified

Specificity: The antibody IVA103 reacts with an extracellular epitope of CD45R antigen (bovine), a single chain type I transmembrane glycoprotein (member of the protein tyrosine phosphatase family), a restricted form of the CD45 family. CD45R is an essential regulator of T and B lymphocytes antigen receptor signaling and it is specifically expressed in hematopoietic cells.

Cross-Reactivity (Details): Bovine

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Bovine leukocytes.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD45 antibody (ABIN94134)

"Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995." in: Veterinary immunology and immunopathology, Vol. 52, Issue 4, pp. 213-468, (1997) (PubMed).

Naessens, Howard, Hopkins: "Nomenclature and characterization of leukocyte differentiation antigens in ruminants." in: Immunology today, Vol. 18, Issue 8, pp. 365-8, (1997) (PubMed).

Sopp, Howard: "Investigation of PC36 (BoCD45R)." in: Veterinary immunology and immunopathology, Vol. 52, Issue 4, pp. 271-4, (1997) (PubMed).

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45R

Background: CD45R, also known as B220, is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.,PTPRCR, T200R

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling