CD45RB antibody

Catalog No. ABIN94146

This anti-CD45RB antibody is a Mouse Monoclonal antibody detecting CD45RB in FACS, WB and IHC (p). Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for CD45RB antibody (ABIN94146)

Target: CD45RB

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RB antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MEM-143

Product Details anti-CD45RB Antibody

Purpose: Anti-Hu CD45RB Purified

Specificity: The antibody MEM-143 reacts with an extracellular epitope of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. The MEM-143 is therefore not neuraminidase sensitive like most other anti-CD45RB antibodies (including standard antibody MEM-55). The reactivity of the antibody MEM-143 can be blocked by a peptide including amino acids 79-88.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human peripheral blood lymphocytes.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 0.5-4 μg/mL. The staining pattern of the MEM-143 shows prominent differences from other anti-CD45RB reagents including standard antibody MEM-55, mostly as a result of the recognition of differences in glycosylation.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive control: human tonsil.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD45RB antibody (ABIN94146)

Koethe, Zander, Köster, Annan, Ebenfelt, Spencer, Bemark: "Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation." in: Journal of leukocyte biology, Vol. 90, Issue 1, pp. 5-19, (2011) (PubMed).

Target Details for CD45RB

Target: CD45RB

Alternative Name: CD45RB

Background: CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.,PTPRCR, T200RB