ICAM1 antibody

Catalog No. ABIN94178

The Mouse Monoclonal anti-ICAM1 antibody is suitable to detect ICAM1 in samples from Human. It has been validated for WB, FACS, ICC, IHC (p) and IHC (fro).

Quick Overview for ICAM1 antibody (ABIN94178)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1H4

Product Details anti-ICAM1 Antibody

Purpose: Anti-Hu CD54 Purified Azide Free

Cross-Reactivity (Details): Other not tested, Human

Characteristics: The antibody 1H4 recognizes an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells, the expression of CD54 is upregulated by activation.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Raji cells and spleen cells fused with NS1 cells

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry (frozen sections): Recommended dilution: 5-10 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, prolonged fixation in buffered formalin can destroy the epitope. High temperature antigen unmasking technique is required.
Flow cytometry: Recommended dilution: 1-4 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL, non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Preservative: Azide free

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: CD54

Background: Intercellular adhesion molecule 1,CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.,ICAM-1, BB2, P3.58

Gene ID: 3383

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling